Yes, three men and two boys at Pottawattamie Creek.
http://www.kancoll.org/khq/1956/56_3_johannsen.htm
Announcing himself to be an instrument of in the hands of God, John Brown and his followers dragged these five from their homes and hacked them to death. That's what Harriet Beecher Stowe had in mind when she wrote about "His terrible swift sword". Remember that next time you sing 'that' song.
You could write it off as the misguided and tragic work of a madman except for the sympathetic reaction of the Northern public to the Pottawattamie Creek massacre and Brown's Harpers Ferry raid. It was similar to hearing about people overseas cheering the news of 9/11.
Here's a example of the kinds of letters written to Gov. Wise of Virginia --
http://www.lva.lib.va.us/whoweare/exhibits/DeathLiberty/johnbrown/anon30.htm
Is it any wonder that Southern volunteer militia companies began organizing during these years, especially after the Harpers Ferry Raid?