Jim, I've sent you a direct email regarding Holt. As to his education, his father attended the U of NC. The lived in middle TN, and for middle Tennesseans the "natural" college to attnd would be that of the U. of Nashville. And in my notes, it says his brother Joseph David Holt (b. 4-15-1835) also was a physician. He also had brothers Lucius and Isaac W. Holt